Showing posts with label The Power of Grafting Series. Show all posts
Showing posts with label The Power of Grafting Series. Show all posts

Wednesday, April 28, 2010

The power of grafting – 6th anniversary

A week ago my grafted Lophophora williamsii (SB 854; Starr Co, Texas) celebrated its 6th anniversary sitting on top a sturdy Trichocereus pachanoi stock. The plant was grafted as a tiny seedling and in 6 years the main head has grown to a width of approximately 8 cm (~3.2'') – the 3 largest offshoots are 6 cm (~2.4'') wide each. The main head seems to have stabilized at 8 ribs while the largest pups are in transition from 5 to 8 ribs.

Lophophora williamsii graft, top view
Lophophora williamsii graft, top view

The last couple of weeks the plant has “popped out” fruits resulting from last years late flowers. This season’s first flower appeared a couple of days ago and was promptly (self-)pollinated – according to previous experience this should result in another fruit in 7-8 weeks time.

Grafted Lophophora williamsii, first flower of the season
Grafted Lophophora williamsii, first flower of the season

Lophophora williamsii flower and fruit
Lophophora williamsii flower and fruit

I’ve already harvested more than 120 seeds from this plant alone this season. As this variety of Lophophora williamsii is not particularly frost hardy I’m not sure if I’ll start all these seeds as the seedlings will have to go to my coldhouse – maybe I should set up a “swap shop” instead ;-)

Lophophora williamsii fruits
Lophophora williamsii fruits

I’m wondering for how long the Trichocereus pachanoi stock will be able to support the rather large Lophophora williamsii scion. This is my oldest graft so I have no experience to rely on regarding the actual longevity of Trichocereus stock plants; I guess time will tell.

Lophophora williamsii grafted on Trichocereus pachanoi stock
Lophophora williamsii grafted on Trichocereus pachanoi stock

For comparison you can check the posts on the same graft as one, two, three, four, and five years old.

Tuesday, April 21, 2009

The power of grafting – 5th anniversary

Five years ago today, I grafted a tiny Lophophora williamsii (SB 854; Starr Co, Tx) seedling onto a robust Trichocereus pachanoi stock. The Lophophora scion has since grown considerably – the main head is approximately 8 cm (~3.2'') wide; the total width including the offshoots now exceeds 14 cm (~5.5'').

Lophophora williamsii grafted onto Trichocereus pachanoi stock
Lophophora williamsii grafted onto Trichocereus pachanoi stock

This growing season the plant hasn't flowered yet but one of the “pups” (new offshoot stems) will do so within days as is evident from the next picture.

Flower bud and red spider mites
Flower bud and red spider mites

Unfortunately not only the plants are returning to life after their winter hibernation – the dreaded red spider mites are also fully active again (you should be able to spot at least four mites in the photo above) so the fight is on once more.

Ripe Lophophora williamsii fruit
Ripe Lophophora williamsii fruit

Even though most of last year's fruits have long since ripened (and been harvested) the odd fruit is still showing. A plant this size gives hundreds of seeds each year making it a virtual seed “factory”.

Lophophora williamsii - rib count increasing?
Lophophora williamsii - rib count increasing?

The plant seems set on increasing its rib count beyond 8 – if I'm lucky it will not be content with growing just a few more ribs but go straight for the next number in the Fibonacci sequence, 13. The more ribs the merrier as it means more areoles and consequently more flowers and seeds.

The main head of the grafted Lophophora williamsii has grown 14 pups (15 if you also count a pup's pup – I guess you could call that a grandpup ;-) and has reached a size where it needs to be repotted soon.

For comparison you can check the posts on the same graft as one, two, three, and four years old.

Sunday, May 04, 2008

Time-lapse video of a flowering Lophophora williamsii

I've been away on a short vacation and it seems my grafted Lophophora williamsii (SB 854; Starr Co, Tx) has spent its "lone time" wisely and grown three flowers.

Lophophora williamsii displaying three flowers
Lophophora williamsii displaying three flowers

Even though the flowers were already quite open when I returned home, I decided to attempt a time-lapse video showing the flowers open fully. Unfortunately I forgot to disable the cameras autofocus function which gives the video a shaky appearance as the camera continuously changes focus slightly.

It seems like the movie couldn't be embedded - you can watch it at http://www.youtube.com/watch?v=wjKgkNyeP68

That being said, I'm impressed by the agility of the Lophophora stamens - they seem almost to be dancing around the style. A dazzling display of thigmotropic behavior is shown 50some seconds into the video; the stamens are disturbed and fold rapidly in around the style only to resume their perpetual wiggle shortly after. In contrast to this Epithelantha micromeris v. greggii stamens never seem to move during flowering.

I yet haven't managed to create a time-lapse video that is fully satisfying (the flower must be shown from bud to full bloom, the image must not shake/autofocus, no drop outs must occur in the stream of images, etc). I'll keep trying though, but I'll bet that when everything is set for the perfect time-lapse the shutter of my good old D70 gives up the ghost just before the shoot is finished ;-)

The photos for the time-lapse were shot at intervals of 15 seconds during a period of 160 minutes. The pictures are played back at a rate of 10 frames per second, i.e. the flowering is speed up by a factor of 150 resulting in a video running for 64 seconds.

Monday, April 21, 2008

The power of grafting – 4th anniversary

Four years ago today, I grafted a tiny Lophophora williamsii (SB 854; Starr Co, Tx) seedling onto a robust Trichocereus pachanoi stock. The Lophophora scion has since grown to a width of 8 cm (~3.2'') and set 6 offshoots - the total width including the offshoots exceeds 13 cm (~5.1'').

Grafted Lophophora williamsii (SB 854; Starr Co, Tx)
Grafted Lophophora williamsii (SB 854; Starr Co, Tx)

The plant flowered throughout summer last year and many of the resulting seed pods are ready to be harvested. This season's first flower already appeared last week and the remains can be seen in the photo below.

Lophophora williamsii - ready for seed harvest
Lophophora williamsii - ready for seed harvest

Last year's flowers were allowed to self-pollinate with minimal human intervention. Today I harvested all freely exposed fruits and left the rest to fully mature. So far the result seems good as the plant set a lot of seed, time will show if it is viable.

Dried Lophophora williamsii fruit
Dried Lophophora williamsii fruit

Many of the fruits have a dry and brittle husk that is easily ripped and some of them almost spilled open when harvested. I probably ought to harvest the seeds sooner and not allow the fruits to sit and dry on the plant for too long.

Ripped Lophophora williamsii fruit with seeds exposed
Ripped Lophophora williamsii fruit with seeds exposed

As mentioned the plant has set lots of (selfed) seeds: the initial harvest totalled 101 seeds and there are still a few fruits left on the plant to be harvested later.

101 home grown Lophophora williamsii seeds
101 home grown Lophophora williamsii seeds

For comparison you can check the posts on the same graft as one, two, and three years old.

Saturday, April 21, 2007

The power of grafting – 3rd anniversary

Today one of my Lophophora williamsii (SB 854; Starr Co, Tx) plants is celebrating its 3rd anniversary as a scion grafted onto a Trichocereus pachanoi stock. The plant has set 4 offshoots and grown to a width of 7.3 cm (~2.9''), 3 of the offshoots are wider than 4 cm (~1.6'') while the smallest just exceeds 2.5 cm (~1'').

Grafted Lophophora williamsii (SB 854; Starr Co, Tx)
Grafted Lophophora williamsii (SB 854; Starr Co, Tx)

The plant set 9 flowers last year and has already displayed two flowers this year. The dried fruits resulting from last season’s flowers are clearly visible in the above photo along with the withered remains of the two “fresh” flowers from this week. The photo below was taken a couple of days ago while the flowers were still at their peak.

Two early Lophophora williamsii flowers
Two early Lophophora williamsii flowers

Usually I assist the plant with its self-pollination but this season I will not interfere. I’m doing this to test to which degree the plant will be able to set seed without any help – of course I can’t do anything about the thigmotropic reactions of the stamens or insects taking liberties with the flowers ;-)

A couple of days ago a flower bud also appeared on the largest of the offshoots and it will probably bloom within a week; unfortunately I will not be home to witness it. If all the offshoots begin to flower and the main shoot continues to flower in its usual pace, I’ll soon grow more seeds than I’ll ever need.

Offshoot setting a flower
Offshoot setting a flower

Since last summer the Trichocereus pachanoi stock has also been allowed to grow an offshoot. Usually I remove these from stock plants in order not to starve the scion, but this one appeared almost at soil level and I decided to let it grow and later graft one of last years Lophophora williamsii (SB 854; Starr Co, Tx) seedlings onto it (these seedlings are the result of self-pollinating the above plant, i.e. technically they are clones of the mother plant). I had planned to do the graft today, but I’ll leave for the Netherlands tomorrow and decided to postpone doing the graft till I’m back.

Trichocereus pachanoi stock with offshoot
Trichocereus pachanoi stock with offshoot

I was in doubt whether or not to include the last photo but decided to do it anyway. I’m fascinated by the dreamy image of the flowers backlit by the soft light of the setting sun, filtered through the foliage of a sprawling Bowiea volubilis. My girlfriend finds the picture boring and “too green” – but who is she to trust, she’s not into cacti ;-)

Flowers backlit by the setting sun
Flowers backlit by the setting sun

For comparison you can check the posts on the same graft written one and two years ago.

Update - April 28, 2007
Fortunately I made it home from the Netherlands in time to experience the pup unfold its first flower. The flower is not as sturdy as those of the main shoot, but what’s to expect from a novice ;-)

Flowering Lophophora williamsii pup
Flowering Lophophora williamsii pup

Today I also removed the dried fruits of last season’s flowers and freed the seeds.

9 dried Lophophora williamsii fruits
9 dried Lophophora williamsii fruits

Close-up of dried Lophophora williamsii fruit
Close-up of dried Lophophora williamsii fruit

The result of the harvest was approximately 100 seeds.

Home grown Lophophora williamsii seeds
Home grown Lophophora williamsii seeds

Saturday, September 30, 2006

Lophophora williamsii – autumn flowers

Having passed the autumnal equinox I hadn’t expected any more flowers from my lophs – nevertheless two of them decided to bloom this week, one for the first time.

Lophophora williamsii (SB 854; Starr Co, Tx) flower
Lophophora williamsii (SB 854; Starr Co, Tx) flower

My grafted Lophophora williamsii (SB 854; Starr Co, Tx) has been flowering abundantly all summer. Several fruits have already ripened and more are budding; still the plant took the opportunity to squeeze in yet another flower before going dormant. The flowers of this plant are quite large, approximately 2.75cm (~1.1'') in diameter.

Lophophora williamsii var. caespitosa flower
Lophophora williamsii var. caespitosa flower

The Lophophora williamsii var. caespitosa graft has been growing profusely but never felt like flowering before now. The flowers are small, less than 2cm (~0.8'') in diameter, compared to those of L. williamsii (SB 854; Starr Co, Tx), and the midstripes are a slightly darker shade of pink.

Lophophora williamsii var. caespitosa
Lophophora williamsii var. caespitosa

The scion used for this graft was taken from the plant shown in the blog profile. Unfortunately this plant later caught rot and died.

Thursday, August 24, 2006

Flowering Lophophora williamsii – III

My Lophophora williamsii (SB 854; Starr Co, Tx) graft has gone on a flowering spree and is currently flashing its 7th and 8th flower this season.

Flowering Lophophora williamsii
Flowering Lophophora williamsii

Like other species of Lophophora, L. williamsii has sensitive stamens, closing around the style when touched.

L. williamsii stamens closing around the style
L. williamsii stamens closing around the style

In this flower the anthers are actually brushing the stigmas when the stamens react to a touch, i.e. the thigmotropic reaction will assist the flower to self-pollinate. I have to test if this is sufficient to pollinate the flower (in a previous post I also wondered if it was necessary to manually self-pollinate the Lophophora williamsii (SB 854; Starr Co, Tx) flowers or if they could cope without any help ;-)

For comparison you can check a photo of thigmotropic behavior in Lophopohora jourdaniana. In this case the stamens fold in below the stigmas, apparently not touching them.

The fruits (or berries, pods, whatever;-) from the plants first two flowers this season have just appeared.

Lophophora williamsii fruits
Lophophora williamsii fruits

I’m looking forward to see how many of the flowers will actually result in a fruit.

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Monday, July 31, 2006

Flowering Lophophora williamsii – II

As mentioned in an earlier post flowering was late this year. My grafted Lophophora williamsii (SB 854; Starr Co, Tx) is now making up for this by showing off its sixth flower this season.

Flowering Lophophora williamsii – 6th flower this season
Flowering Lophophora williamsii – 6th flower this season

The three previous flowers unfolded while I was away on vacation – the remains of all the spent flowers are visible beneath the current one.

This flowering coincided with the flowering of a Lophophora williamsii v. jourdaniana. I decided not to outcross the plants but instead self-pollinate both of them; first of all to keep my SB 854’s “clean”, secondly I want to test the alleged self-incompatibility of the jourdaniana variety.

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Friday, July 14, 2006

Flowering Lophophora williamsii

Flowering has been late this year but finally my grafted Lophophora williamsii (SB 854; Starr Co, Tx) decided to come back with a vengeance by setting two flowers simultaneously.

Lophophora williamsii with two flowers
Lophophora williamsii with two flowers

The scar visible in the upper left-hand corner of the photo formed after the epidermis ripped, caused by too vigorous growth.

The flowers are funnelform, pinkish white with a light pink midstripe.

Funnelform Lophophora flowers reaching for the sun
Funnelform flowers reaching for the sun

I self-pollinated the flowers but am wondering if it’s necessary – the anthers are virtually brushing the stigmas, depositing pollen without any help.

Lophophora anthers brushing stigmas
Anthers brushing stigmas

Last growing season I also posted on this plants flowering and fruit setting (use the previous and next links to navigate the posts).

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Friday, April 21, 2006

The power of grafting - IX (full circle)

It’s time to shake off the dust of the prehistoric peyote buttons from the previous post and get started with the new growing season. Two years ago today I grafted a 50some days old Lophophora williamsii (SB 854; Starr Co, Tx) seedling onto a (then extravagantly oversized) Trichocereus pachanoi stock.

Grafted Lophophora williamsii (SB 854; Starr Co, Tx)
Grafted Lophophora williamsii (SB 854; Starr Co, Tx)

The plant has set 3 offshoots and grown to a width of 5.6 cm (~ 2.2'' – excluding the offshoots) and a height of 4.8 cm (~ 1.9''). It’s currently busy increasing the number of ribs from 5 to 8 (or maybe it goes directly for 13 ribs... it’s a bit hard to tell at this stage).

For comparison you can check this photo of the same graft one year ago.

Full circle
The plant flowered 4 times last year – all flowers were self-pollinated. The resulting fruits contained 22 seeds that were sown April 4, but until now only 5 have germinated and out of these only 3 seedlings are thriving. Yet another illustration of why inbreeding should be avoided.

Lophophora seedling grown from self-pollinated seeds
Lophophora seedling grown from self-pollinated seeds

I find it quite amazing that grafting enables you to go full circle from seedling to seedling in two years.

A more detailed account of the plant’s flowering and fruit setting can be had by following the “previous” links.

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Wednesday, February 15, 2006

The power of grafting – VIII

My grafted Lophophora williamsii (SB 854; Starr Co, Tx) is growing its last fruit this season. The fruit looks ripe and fat so I'm hoping for lots of seeds even though the flower was self-pollinated.

Lophophora williamsii - ripe fruit
Lophophora williamsii - ripe fruit

Yesterday I harvested the dried fruits produced by the plants second and third flower – both of these flowers were also self-pollinated (I didn’t have a whole lot of plants flowering last year ;-)

Lophophora williamsii - harvested fruits
Lophophora williamsii - harvested fruits

The fruits are approximately 1 cm (~0.4'') long, only the upper half is containing seeds. The yield from these fruits, each contained 6 seeds, is much better than from the first fruit I harvested.

Lophophora williamsii - seeds
Lophophora williamsii - seeds

If the seeds are viable I hope to come full circle, growing new seedlings in less than two years after the mother plant was sown!

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Saturday, November 26, 2005

The power of grafting – VII

Most of my plants are now dormant and I hadn’t expected much activity to report on these pages – but 5 days ago I noticed a budding fruit on my grafted Lophophora williamsii (SB 854; Starr Co, Tx).

Budding Lophophora williamsii fruit
Budding Lophophora williamsii fruit

The fruit is now ripe and slightly longer than 1 cm (~ 0.4’’) with a diameter of 4.5 mm (~ 0.2’’) at the widest section. The color is best described as a pinkish magenta segueing into a lighter pink on the surface not directly exposed to the sun.

Lophophora williamsii with ripe fruit
Lophophora williamsii with ripe fruit

Lophophora williamsii fruit – close-ups
Lophophora williamsii fruit – close-ups

While examining the plant it became apparent that another fruit is breaking out. If this one ripens as abruptly as the last, it will be fully extruded within a week.

Fruiting Lophophora williamsii
Fruiting Lophophora williamsii

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Sunday, October 23, 2005

The power of grafting - VI

Today I harvested the first seeds from my grafted Lophophora williamsii (SB 854; Starr Co, Tx). The fruit appeared mid-September and has been maturing on the plant for some weeks. The photo below shows the fruit on September 17.

Lophophora williamsii fruit
Lophophora williamsii fruit

The fruit has been dry and ripe for picking for a while now – as illustrated by the next photo, taken just before the fruit was removed.

Dry Lophophora williamsii fruit
Dry Lophophora williamsii fruit

I blame it on the self-fertilization; the fruit only contained 3 seeds that looked OK and 2 that weren’t fully developed. It will be interesting to see if any of the seeds are viable.

Lophophora williamsii seeds
Lophophora williamsii seeds


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Monday, September 12, 2005

The power of grafting - V

My grafted Lophophora williamsii (SB 854; Starr Co, Tx) is on a roll – it is now flowering for the fourth time this year.

Lophophora williamsii flower
Lophophora williamsii flower

I self-pollinated the flower and collected some pollen for a Lophophora williamsii v. jourdaniana that will flower soon – allegedly this variety is self-incompatible i.e. it requires outcross pollination. I hope the pollen will stay viable in the refrigerator for a week or two.

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Monday, August 29, 2005

The power of grafting - IV

For the third time this year my grafted Lophophora williamsii (SB 854; Starr Co, Tx) is flowering – quite an accomplishment from a plant only 18 months from seed (sowed February 29, 2004). This flower was also self-pollinated.

Flowering Lophophora williamsii
Flowering Lophophora williamsii

The two spent flowers can be seen below the current one.

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Monday, August 22, 2005

The power of grafting - III

My grafted Lophophora williamsii (SB 854; Starr Co, Tx) is flowering again (and yet another bud is in the works). Once again I had to self-pollinate the flower since none of my other plants are blooming.

Flowering Lophophora williamsii
Flowering Lophophora williamsii

Growth will tear us apart
The scion is growing so hard you can practically hear the epidermis squeaking. A couple of days ago it gave in to the pressure and burst – maybe triggered by the plant “rearranging itself”, starting to grow more ribs. The scion has grown to a width of 5 cm (~1.97’’), excluding the offshoots.

Bursting Lophophora williamsii.
Bursting Lophophora williamsii

The Echinopsis (Trichocereus) pachanoi stock refuses to give up the fight and is stubbornly growing yet another offshoot.

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